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Nuclear Antigen (Pan-Nuclear Marker) anticorps

L’anticorps Souris Monoclonal anti- a été validé pour IHC, ISt, StM, FACS et IF. Il convient pour détecter dans des échantillons de Humain, Souris et Rat.
N° du produit ABIN6938094

Aperçu rapide pour Nuclear Antigen (Pan-Nuclear Marker) anticorps (ABIN6938094)

Antigène

Nuclear Antigen (Pan-Nuclear Marker)

Reactivité

Humain, Souris, Rat

Hôte

  • 2
  • 1
Souris

Clonalité

  • 3
Monoclonal

Conjugué

  • 3
Inconjugué

Application

Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone

NM106
  • Specificité

    This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

    Aucune reactivité croisée

    Porc

    Purification

    Purified by Protein A/G

    Immunogène

    Nuclei of HL60 cells

    Isotype

    IgG1 kappa
  • Indications d'application

    Positive Control: All human cells. Tonsil.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions

    For Research Use only
  • Buffer

    10 mM PBS without BSA and without Azide.

    Agent conservateur

    Azide free

    Stock

    4 °C,-80 °C

    Stockage commentaire

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

    Date de péremption

    24 months
  • Antigène

    Nuclear Antigen (Pan-Nuclear Marker)
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