PAX8 anticorps (AA 60-261)
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- Antigène Voir toutes PAX8 Anticorps
- PAX8 (Paired Box 8 (PAX8))
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Épitope
- AA 60-261
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp PAX8 est non-conjugé
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- Recognizes a protein of 62 kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.
- Réactivité croisée (Details)
- Human. Dog.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Recombinant fragment (around aa 60-261) of human PAX8 protein (exact sequence is proprietary)
- Clone
- PAX8-1492
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product PAX8 Anticorps primaire
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- Indications d'application
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Raji cells. Renal Cell Carcinoma (RCC).
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- PAX8 (Paired Box 8 (PAX8))
- Autre désignation
- PAX8 (PAX8 Produits)
- Synonymes
- anticorps PAX8, anticorps XPax-8, anticorps XPax8, anticorps pax-8, anticorps PAX-8, anticorps Pax-8, anticorps paired box 8, anticorps paired box 8 L homeolog, anticorps PAX8, anticorps pax8, anticorps Pax8, anticorps pax8.L
- Sujet
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Paired box 8, Paired box gene 8, paired box homeotic gene 8, Paired box protein Pax-8, Paired domain gene 8, PAX8,PAX8 (Renal Cell Marker)
Cellular localisation: Nuclear - Poids moléculaire
- 62kDa
- ID gène
- 7849, 469728
- UniProt
- Q06710
- Pathways
- Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation
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