PDLIM1 anticorps

N° du produit ABIN6938142

L’anticorps Souris Monoclonal anti-PDLIM1 a été validé pour WB et IHC (f). Il convient pour détecter PDLIM1 dans des échantillons de Humain.

Aperçu rapide pour PDLIM1 anticorps (ABIN6938142)

Antigène: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDLIM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CPTC-PDLIM1-1

Détail du produit anti-PDLIM1 anticorps

Specificité: CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88 % sequence similarity with its rat counterpart, suggesting a conserved function between species.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human full-length PDLIM1 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 cells and extracts from Jurkat. Human heart, colon or spleen.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur PDLIM1

Antigène: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Autre désignation: PDLIM1

Sujet: C terminal LIM domain protein 1, carboxy terminal LIM domain protein 1, CLIM1, CLP36, Elfin, Enigma antibody, Enigma homolog antibody, hCLIM1, PDLIM1, PDZ and LIM domain 1,PDZ and LIM Domain 1
Cellular localisation: Cytoplasm.

Poids moléculaire: 36kDa

ID gène: 9124, 368525

UniProt: O00151