Recombinant POMC anticorps (N-Term)

N° du produit ABIN6938217

Détail du produit anti-POMC anticorps

Antigène: POMC (Proopiomelanocortin (POMC))

Type d'anticorp: Recombinant Antibody

Épitope: N-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp POMC est non-conjugé

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Réactivité croisée (Details): Human, Mouse and Rat. Expected to show a broad species reactivity.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: N-terminal fragment of human ACTH conjugated to KLH

Clone: R57

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Normal pituitary gland or pituitary tumor.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur POMC

Antigène: POMC (Proopiomelanocortin (POMC))

Autre désignation: POMC (POMC Produits)

Synonymes: anticorps MSH, anticorps ACTH, anticorps pomc, anticorps SI:bZ36D5.1, anticorps SI:bZ36D5.4, anticorps si:bz36d5.2, anticorps BE, anticorps Beta-LPH, anticorps Clip, anticorps Gamma-LPH, anticorps Npp, anticorps Pomc-1, anticorps Pomc1, anticorps alpha-MSH, anticorps alphaMSH, anticorps beta-MSH, anticorps gamma-MSH, anticorps CLIP, anticorps LPH, anticorps NPP, anticorps POC, anticorps Pomc2, anticorps proopiomelanocortin a, anticorps pro-opiomelanocortin-alpha, anticorps proopiomelanocortin, anticorps pomca, anticorps Pomc, anticorps POMC

Sujet: Adrenocorticotropin, alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin, beta-Endorphin, beta or gamma Lipotropin (LPH), CLIP, Met Enkephalin, POC, POMC,ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker)
Cellular localisation: Cytoplasm. Secreted.

Poids moléculaire: ACTH is ~5kDa, and the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

ID gène: 5443, 1897

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour