SOX10 anticorps (AA 115-269)

N° du produit ABIN6938404

Détail du produit anti-SOX10 anticorps

Antigène: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Épitope: AA 115-269

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SOX10 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Réactivité croisée (Details): Human. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary)

Clone: SOX10-991

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: COLO-38 or HepG2 cells. Melanomas, breast carcinomas, gliomas.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur SOX10

Antigène: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Autre désignation: SOX10 (SOX10 Produits)

Synonymes: anticorps DOM, anticorps PCWH, anticorps WS2E, anticorps WS4, anticorps WS4C, anticorps SOX-10, anticorps SOX10, anticorps dom, anticorps ws4, anticorps Dom, anticorps Sox21, anticorps SOX9, anticorps cls/sox10, anticorps zgc:100757, anticorps SRY-box 10, anticorps SRY box 10, anticorps SRY-box 10 L homeolog, anticorps SRY (sex determining region Y)-box 10, anticorps SOX10, anticorps Sox10, anticorps sox10.L, anticorps sox10

Sujet: DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E(WS2E), WS4, Waardenburg syndrome type 4C (WS4C),SOX10 (Melanoma Marker)
Cellular localisation: Nuclear

Poids moléculaire: 49-58kDa

ID gène: 6663, 376984

UniProt: P56693

Pathways: Chromatin Binding