SPTA1 anticorps (AA 356-475)

N° du produit ABIN6938437

Cet anticorps anti-SPTA1 est un anticorps Souris Monoclonal détectant SPTA1 dans WB, ELISA, Coat et IHC (f). Adapté pour Humain.

Aperçu rapide pour SPTA1 anticorps (AA 356-475) (ABIN6938437)

Antigène: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SPTA1 est non-conjugé

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SPTA1-1832

Détail du produit anti-SPTA1 anticorps

Épitope: AA 356-475

Specificité: Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human SPTA fragment (around aa356-475) (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL). Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K562, HeLa, Jurkat cells. Placenta or Tonsil.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur SPTA1

Antigène: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Autre désignation: SPTA1

Sujet: Alpha I spectrin, Elliptocytosis 2 (EL2), Erythrocyte alpha spectrin, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha erythrocytic 1, SPH3, SPTA1,Spectrin Alpha 1 (Erythrocyte Marker)
Cellular localisation: Cytoplasmic and Cell Surface

Poids moléculaire: 240kDa

ID gène: 6708, 119825

UniProt: P02549

Pathways: Regulation of Actin Filament Polymerization