Spectrin beta Chain, Erythrocyte (SPTB) anticorps

N° du produit ABIN6938442

Détail du produit

Antigène: Spectrin beta Chain, Erythrocyte (SPTB)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Coating (Coat), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

Réactivité croisée (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Red cell membranes

Clone: RG-26

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détail du antigène

Antigène: Spectrin beta Chain, Erythrocyte (SPTB)

Autre désignation: SPTB (SPTB Produits)

Synonymes: anticorps AI842465, anticorps D330027P03Rik, anticorps Gm1301, anticorps Spnb-1, anticorps Spnb1, anticorps ja, anticorps jaundiced, anticorps mKIAA4219, anticorps EL3, anticorps HS2, anticorps HSPTB1, anticorps SPH2, anticorps spectrin beta, erythrocytic, anticorps spectrin, beta, erythrocytic, anticorps SPTB, anticorps Sptb

Sujet: Beta-I spectrin, EL3, erythrocyte, HS2, SPTB1, HSPTB1, membrane cytoskeletal protein, spectrin beta (erythrocytic),Spectrin beta I (SPTB)
Cellular localisation: Cell Surface

Poids moléculaire: 246kDa

ID gène: 6710, 26915

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization