Spectrin beta Chain, Erythrocyte (SPTB) anticorps

N° du produit ABIN6938442

L’anticorps anti-Spectrin beta Chain, Erythrocyte Monoclonal Souris est utilisé pour la détection de Spectrin beta Chain, Erythrocyte dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, IF et ELISA.

Aperçu rapide pour Spectrin beta Chain, Erythrocyte (SPTB) anticorps (ABIN6938442)

Antigène: Spectrin beta Chain, Erythrocyte (SPTB)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Clone: RG-26

Détail du produit

Fonction: Spectrin beta I (SPTB) Antibody

Specificité: Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

Réactivité croisée (Details): Human. Mouse. Rat.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Red cell membranes

Isotype: IgG2a, kappa

Information d'application

Indications d'application:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: Spectrin beta Chain, Erythrocyte (SPTB)

Autre désignation: SPTB

Sujet:

beta-I spectrin, EL3, erythrocyte, HS2, SPTB1, HSPTB1, membrane cytoskeletal protein, spectrin beta (erythrocytic)

Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Poids moléculaire: 246kDa

ID gène: 6710, 26915

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization