EpCAM anticorps (Extracellular Domain)

N° du produit ABIN6938479

Cet anticorps anti-EpCAM Monoclonal Souris (Clone EGP40-1798) (ABIN6938479) détecte spécifiquement EpCAM dans WB, FACS, IHC, IF et ELISA. L’anticorps est réactif avec des échantillons de Humain, Chien et Cat.

Aperçu rapide pour EpCAM anticorps (Extracellular Domain) (ABIN6938479)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain, Chien, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Clone: EGP40-1798

Détail du produit anti-EpCAM anticorps

Épitope: AA 100-224, Extracellular Domain

Fonction: Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody

Specificité: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Réactivité croisée (Details): Human. Dog. Cat. Others not known.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 orHT29 cells. Human breast or ovariancarcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: TACSTD1

Sujet:

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, Membrane Component, Chromosome 17, Surface Marker-1 (M17S1), REG-1, Reggie-1, Reggie-2

MCF-7 orHT29 cells. Human breast or ovariancarcinoma.

Poids moléculaire: 40-43kDa

ID gène: 4072, 542050

UniProt: P16422