TAG-72 / CA72.4 anticorps

N° du produit ABIN6938501

Cet anticorps Souris Monoclonal détecte spécifiquement dans FACS, IF, IHC et StM. Il présente une réactivité envers Humain, Chien, Boeuf (Vache), Rat et Hamster.

Aperçu rapide pour TAG-72 / CA72.4 anticorps (ABIN6938501)

Antigène: TAG-72 / CA72.4

Reactivité: Humain, Chien, Boeuf (Vache), Rat, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: B72-3

Détail du produit

Specificité: Recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.

Purification: Purified by Protein A/G

Immunogène: Membrane-enriched fraction of a human breast carcinoma liver metastasis

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Jurkat cells, Breast or lung carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Buffer: 10 mM PBS without BSA and without Azide.

Agent conservateur: Azide free

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Date de péremption: 24 months

Détail du antigène

Antigène: TAG-72 / CA72.4

Autre désignation: TAG-72 / CA72.4 (Tumor-Associated Glycoprotein)

Poids moléculaire: 220kDa

ID gène: 182875