Transgelin anticorps

N° du produit ABIN6938512

Cet anticorps anti-Transgelin est un anticorps Souris Monoclonal détectant Transgelin dans FACS, IF et IHC (f). Adapté pour Humain.

Aperçu rapide pour Transgelin anticorps (ABIN6938512)

Antigène: Transgelin (TAGLN)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transgelin est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SPM606

Détail du produit anti-Transgelin anticorps

Specificité: This MAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

Réactivité croisée (Details): Human. Cow. Pig. Rabbit. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human transgelin (TAGLN) protein.

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Transgelin

Antigène: Transgelin (TAGLN)

Autre désignation: TAGLN

Sujet: 2 kDa actin-binding protein, Human 22 kDa smooth muscle protein, Protein WS3-10, SMCC, Smooth muscle protein 22-alpha (SM22-alpha), TAGLN, TAGLN1, transgelin, Transgelin variant 2, WS3-10,Transgelin (SM22-alpha)
Cellular localisation: Cytoplasmic

Poids moléculaire: 22kDa

ID gène: 6876, 410977

UniProt: Q01995