p63 anticorps (AA 5-17)

N° du produit ABIN6938658

L’anticorps anti-p63 Polyclonal Lapin est utilisé pour la détection de p63 dans des échantillons de Humain, Souris, Rat et Boeuf (Vache). Il a été validé pour WB et IHC.

Aperçu rapide pour p63 anticorps (AA 5-17) (ABIN6938658)

Antigène: p63 (TP63) (Tumor Protein P63 (TP63))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp p63 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-p63 anticorps

Épitope: AA 5-17

Fonction: p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) Antibody

Specificité: P40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Réactivité croisée (Details): Human. Mouse. Rat. Cow.

Purification: 200ug/ml of Ab Purified from rabbit anti-serum by Protein A.

Immunogène: A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: HEK293 cells. Prostate Carcinoma or Lung Squamous Cell Carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur p63

Antigène: p63 (TP63) (Tumor Protein P63 (TP63))

Autre désignation: TP63

Sujet:

deltaNp63, TP63

HEK293 cells. Prostate Carcinoma or Lung Squamous Cell Carcinoma.

Poids moléculaire: 40kDa

ID gène: 8626, 137569

UniProt: Q9H3D4