TYMS anticorps

N° du produit ABIN6938689

Détail du produit anti-TYMS anticorps

Antigène: TYMS (Thymidylate Synthetase (TYMS))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TYMS est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Radioimmunoassay (RIA)

Specificité: It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human TYMS protein.

Clone: TS106

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur TYMS

Antigène: TYMS (Thymidylate Synthetase (TYMS))

Autre désignation: TYMS (TYMS Produits)

Synonymes: anticorps FN1, anticorps ECK2823, anticorps JW2795, anticorps ts, anticorps Ts, anticorps HST422, anticorps TMS, anticorps TS, anticorps thymidylate synthetase, anticorps contains intron in T4 phage, anticorps ThyE, anticorps thymidylate synthase, anticorps thymidylate synthetase L homeolog, anticorps TYMS, anticorps thyA, anticorps td, anticorps thyE, anticorps DDA3937_RS04960, anticorps tyms.L, anticorps XBJ1_RS15770, anticorps EAMY_RS20680, anticorps Tyms

Classe de substances: Viral Protein

Sujet: DTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase,Thymidylate Synthase (5-FU Resistance Marker)
Cellular localisation: Cytoplasm. Nucleus. Mitochondria.

Poids moléculaire: 36kDa

ID gène: 7298, 369762

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases