Villin 1 anticorps (AA 179-311)

N° du produit ABIN6938747

Cet anticorps Souris Monoclonal détecte spécifiquement Villin 1 dans WB et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour Villin 1 anticorps (AA 179-311) (ABIN6938747)

Antigène: Villin 1 (VIL1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Villin 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: VIL1-1314

Détail du produit anti-Villin 1 anticorps

Épitope: AA 179-311

Specificité: Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549, HepG2 and HCT116 cells. Colon or Rectum.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Villin 1

Antigène: Villin 1 (VIL1)

Autre désignation: VIL1

Sujet: VIL1, Villin-1, Villin1,Villin (GI-Mucosal & Urogenital Brush Border Marker)
Cellular localisation: Cytoplasmic & Cell Surface

Poids moléculaire: 93kDa

ID gène: 7429, 654595

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization