WT1 anticorps (AA 1-181)

N° du produit ABIN6938782

Cet anticorps anti-WT1 est un anticorps Souris Monoclonal détectant WT1 dans IHC (f). Adapté pour Humain.

Aperçu rapide pour WT1 anticorps (AA 1-181) (ABIN6938782)

Antigène: WT1 (Wilms Tumor 1 (WT1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp WT1 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 6F-H2

Détail du produit anti-WT1 anticorps

Épitope: AA 1-181

Specificité: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Réactivité croisée (Details): Human. Rat. Weakly reacts with Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant protein corresponding to residues 1-181 of human WT1.

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Positive_Control: K562 cells. Kidney, Testis, Wilm's Tumor or Mesothelioma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur WT1

Antigène: WT1 (Wilms Tumor 1 (WT1))

Autre désignation: WT1

Sujet: WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1,Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)
Cellular localisation: Nuclear and Cytoplasmic

Poids moléculaire: 47-55kDa

ID gène: 7490, 591980

UniProt: P19544

Pathways: Tube Formation