N-Cadherin anticorps (Intracellular)

N° du produit ABIN6939014

Cet anticorps anti-N-Cadherin est un anticorps Souris Monoclonal détectant N-Cadherin dans IHC (f). Adapté pour Humain.

Aperçu rapide pour N-Cadherin anticorps (Intracellular) (ABIN6939014)

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp N-Cadherin est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CDH2-1573

Détail du produit anti-N-Cadherin anticorps

Épitope: Intracellular

Specificité: Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. Results aid in the classification of malignant non-carcinomatous neoplasms including mesotheliomas, chordomas, synovial sarcomas, malignant melanomas, epithelioid sarcomas, epithelioid angiosarcomas, clear cell sarcomas as well as serous and endometrioid tumors of the ovary have been demonstrated to be N-cadherin positive, whereas mucinous tumors are negative. Other N-cadherin-positive neoplasms include renal cell carcinomas and some variant breast tumors, including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas.

Réactivité croisée (Details): Human, Mouse,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human CDH2 intracellular domain

Isotype: IgG1

Information d'application

Indications d'application:

Positive Control: HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur N-Cadherin

Antigène: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Autre désignation: CDH2

Sujet: Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD,N-Cadherin / Cadherin-2 / CD325 (NCAD)
Cellular localisation: Cell surface

Poids moléculaire: 130-140kDa

ID gène: 1000, 464829

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane