DMC1 anticorps

N° du produit ABIN6939141

Cet anticorps Souris Monoclonal détecte spécifiquement DMC1 dans WB, IHC, IF et StM. Il présente une réactivité envers Humain, Souris, Rat et Boeuf (Vache).

Aperçu rapide pour DMC1 anticorps (ABIN6939141)

Antigène: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DMC1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Clone: 2H12-4

Détail du produit anti-DMC1 anticorps

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human DMC1 protein.

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Cervix or prostate.

Known Application: Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur DMC1

Antigène: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Autre désignation: DMC1

Sujet: DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the doublestrand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Nibrin and Dmc1. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients. Dmc1 is specifically involved in meiotic recombination. An alternative spliced form of Dmc1, designated Dmc1-D, is deleted for a region between the two motifs involved in nucleotide binding. The alternatively spliced Dmc1-D transcript is detected in both male and female germ cells, indicating that the encoded protein may have a role in mammalian genetic recombination in meiosis.

Poids moléculaire: 37kDa

ID gène: 11144

UniProt: Q14565