CKB anticorps

N° du produit ABIN6939145

L’anticorps anti-CKB Monoclonal Souris est utilisé pour la détection de CKB dans des échantillons de Humain, Rat, Souris, Cobaye et Hamster. Il a été validé pour WB et IHC.

Aperçu rapide pour CKB anticorps (ABIN6939145)

Antigène: CKB (Creatine Kinase, Brain (CKB))

Reactivité: Humain, Rat, Souris, Cobaye, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CKB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CPTC-CKB-2

Détail du produit anti-CKB anticorps

Fonction: Creatine Phosphokinase-BB (CK-BB) Antibody

Specificité: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human full-length CKB protein

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Positive Control: SH-SY5Y, HEK-293 and Cerebellum.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CKB

Antigène: CKB (Creatine Kinase, Brain (CKB))

Autre désignation: CKB

Sujet:

BCK, Brain creatine kinase, Ckb, Creatine kinase B chain, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine phosphokinase BB, Epididymis luminal protein 211, Epididymis secretory protein Li 29, HEL 211

Y79 or HEK-293T cells. Human cerebellum. Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach or Guinea pig Stomach tissue

Poids moléculaire: 42kDa

ID gène: 1152, 173724

UniProt: P12277