Cathepsin D anticorps (AA 104-250)

N° du produit ABIN6939218

L’anticorps anti-Cathepsin D Monoclonal Souris est utilisé pour la détection de Cathepsin D dans des échantillons de Humain. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour Cathepsin D anticorps (AA 104-250) (ABIN6939218)

Antigène: Cathepsin D (CTSD)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cathepsin D est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: CTSD-3276

Détail du produit anti-Cathepsin D anticorps

Épitope: AA 104-250

Fonction: Cathepsin D (Tumor Marker) Antibody

Specificité: Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins. It is synthesized as an inactive 52 kDa preprocathepsin D that is cleaved and glycosylated to form a 48 kDa procathepsin D and then further cleaved to produce 34 kDa and 14 kDa subunits (heavy and light chains, respectively). Cathepsin D exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment of human Cathepsin D protein (around aa 104-250) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Positive Control: MCF-7, K-562, A431 or SK-BR3 cells. Kidney, liver, lung or breast.

Known Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cathepsin D

Antigène: Cathepsin D (CTSD)

Autre désignation: CTSD

Sujet:

CatD, Cathepsin D, Ceroid lipofuscinosis neuronal 10, CLN10, CPSD, Epididymis secretory sperm binding protein Li 130P, Lysosomal aspartyl peptidase, Lysosomal aspartyl protease

K-562 or SK-BR3 cells. Human kidney, Liver, lung or breast tissue., MCF-7

Poids moléculaire: 48kDa (Procathepsin D), 34kDa (Cathepsin D), 14kDa (Cathepsin D)

ID gène: 1509, 654447

UniProt: P07339

Pathways: Peptide Hormone Metabolism