alpha Fetoprotein anticorps
Aperçu rapide pour alpha Fetoprotein anticorps (ABIN6939237)
Antigène
Voir toutes alpha Fetoprotein (AFP) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Fonction
- AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody
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Specificité
- It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
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Aucune reactivité croisée
- Boeuf (Vache), Souris, Rat (Rattus)
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Purification
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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Immunogène
- Alpha feto protein (AFP) Purified from serum of a hepatoma patient
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Isotype
- IgG2a, kappa
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Indications d'application
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Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
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Restrictions
- For Research Use only
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Concentration
- 200 μg/mL
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Buffer
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Date de péremption
- 24 months
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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Autre désignation
- AFP
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Sujet
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Alpha fetoglobulin, FETA, HPAFP
Hep-G2 cells. Fetal liver or hepatocellular carcinoma.
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Poids moléculaire
- 70kDa
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ID gène
- 174, 518808
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UniProt
- P02771
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Pathways
- C21-Steroid Hormone Metabolic Process
Antigène
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