Dystrophin anticorps (AA 114-263)

N° du produit ABIN6939241

Cet anticorps Souris Monoclonal détecte spécifiquement Dystrophin dans IHC et ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Dystrophin anticorps (AA 114-263) (ABIN6939241)

Antigène: Dystrophin (DMD)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Dystrophin est non-conjugé

Application: Immunohistochemistry (IHC), ELISA

Clone: DMD-3241

Détail du produit anti-Dystrophin anticorps

Épitope: AA 114-263

Fonction: Dystrophin (DMD) (Marker of Duchenne and Becker Muscular Dystrophy) Antibody

Specificité: Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2.4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least 8 independent tissue-specific promoters and 2 poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0.002 % of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: A recombinant fragment (around aa 114-263) of human DMD protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: Human skeletal muscle and heart muscle tissues (IHC).

Known Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Dystrophin

Antigène: Dystrophin (DMD)

Autre désignation: DMD

Sujet:

BMD, CMD3B, Duchenne muscular dystrophy (DMD), Dystrophin, Muscular dystrophy Duchenne and Becker types

Human skeletal muscle and heart muscle tissues (IHC).

Poids moléculaire: 427kDa

ID gène: 1756, 495912

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development