Desmocollin 2 anticorps (Extracellular Domain)

N° du produit ABIN6939250

L’anticorps Souris Monoclonal anti-Desmocollin 2 a été validé pour WB, IHC, IF, FACS et StM. Il convient pour détecter Desmocollin 2 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Desmocollin 2 anticorps (Extracellular Domain) (ABIN6939250)

Antigène: Desmocollin 2 (DSC2)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmocollin 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: 7G6

Détail du produit anti-Desmocollin 2 anticorps

Épitope: Extracellular Domain

Purification: Purified by Protein A/G

Immunogène: Human desmocollin-2 extracellular domain

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Skin tissue.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Desmocollin 2

Antigène: Desmocollin 2 (DSC2)

Autre désignation: DSC2

Sujet: Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers.

Poids moléculaire: 110kDa (DSC2), 100kDa (DSC3)

ID gène: 1824

UniProt: Q02487