Recombinant Thymidine Phosphorylase anticorps

N° du produit ABIN6939278

Cet anticorps Lapin Monoclonal détecte spécifiquement Thymidine Phosphorylase dans IHC et StM. Il présente une réactivité envers Humain, Rat et Souris.

Aperçu rapide pour Recombinant Thymidine Phosphorylase anticorps (ABIN6939278)

Antigène: Thymidine Phosphorylase (TYMP)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Thymidine Phosphorylase est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: TYMP-2890R

Détail du produit anti-Thymidine Phosphorylase anticorps

Specificité: Recognizes a protein (amino acid 482) of 55 kDa (in vivo 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5'-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: HUVEC cells. Breast, Bladder, Lung or Kaposi tumors.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Thymidine Phosphorylase

Antigène: Thymidine Phosphorylase (TYMP)

Autre désignation: TYMP

Poids moléculaire: 55kDa

ID gène: 1890

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2