Estrogen Receptor alpha anticorps (AA 2-185)

N° du produit ABIN6939348

Cet anticorps anti-Estrogen Receptor alpha est un anticorps Souris Monoclonal détectant Estrogen Receptor alpha dans WB, IHC, FACS et StM. Adapté pour Humain.

Aperçu rapide pour Estrogen Receptor alpha anticorps (AA 2-185) (ABIN6939348)

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Estrogen Receptor alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Clone: ER506

Détail du produit anti-Estrogen Receptor alpha anticorps

Épitope: AA 2-185

Specificité: This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (around aa 2-185) of human Estrogen Receptor alpha protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MCF-7 cells. Breast or Endometrial Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), ,Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) ,Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Estrogen Receptor alpha

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Autre désignation: ESR1

Poids moléculaire: ~67kDa

ID gène: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly