Filaggrin anticorps (AA 998-1104)

N° du produit ABIN6939385

Cet anticorps anti-Filaggrin est un anticorps Souris Monoclonal détectant Filaggrin dans IHC, ISt et StM. Adapté pour Humain.

Aperçu rapide pour Filaggrin anticorps (AA 998-1104) (ABIN6939385)

Antigène: Filaggrin (FLG)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Filaggrin est non-conjugé

Application: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Clone: FLG-1563

Détail du produit anti-Filaggrin anticorps

Épitope: AA 998-1104

Purification: Purified by Protein A/G

Immunogène: Recombinant human Filaggrin protein fragment (around aa 998-1104) (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: U-251-MG cells. Skin.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Filaggrin

Antigène: Filaggrin (FLG)

Autre désignation: FLG

Sujet: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Poids moléculaire: 26-45kDa (Processed), 350kDa (Profilaggrin)

ID gène: 2312

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance