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Elastase 3B anticorps (AA 82-238)

ELA3B Reactivité: Humain, Souris, Rat WB, ELISA, Coat, IHC, StM Hôte: Souris Monoclonal CELA3B-1257 unconjugated
N° du produit ABIN6939418
  • Antigène Voir toutes Elastase 3B (ELA3B) Anticorps
    Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
    Épitope
    • 53
    • 9
    • 7
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 82-238
    Reactivité
    • 80
    • 19
    • 18
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 54
    • 35
    Souris
    Clonalité
    • 58
    • 31
    Monoclonal
    Conjugué
    • 42
    • 15
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    Cet anticorp Elastase 3B est non-conjugé
    Application
    • 60
    • 59
    • 44
    • 40
    • 39
    • 38
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (IHC), Staining Methods (StM)
    Specificité
    This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
    Purification
    Purified by Protein A/G
    Immunogène
    Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)
    Clone
    CELA3B-1257
    Isotype
    IgG1 kappa
  • Indications d'application

    Positive Control: Panc-28 or PANC1 cells. Pancreas.

    Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA)Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
    Autre désignation
    CELA3B (ELA3B Produits)
    Synonymes
    anticorps CBPP, anticorps E1, anticorps EL-1, anticorps ELA3B, anticorps 0910001F22Rik, anticorps 2310074F01Rik, anticorps AI504000, anticorps Ela3, anticorps Ela3b, anticorps ela3b, anticorps MGC82867, anticorps ELA1, anticorps Cela3b, anticorps CELA3B, anticorps chymotrypsin like elastase family member 3B, anticorps chymotrypsin-like elastase family, member 3B, anticorps elastase 3B, pancreatic, anticorps proproteinase E, anticorps chymotrypsin like elastase family member 3B L homeolog, anticorps chymotrypsin-like elastase family member 3B, anticorps CELA3B, anticorps Cela3b, anticorps ela3b, anticorps LOC478196, anticorps cela3b.L, anticorps LOC100717936, anticorps LOC100071692
    Poids moléculaire
    ~17kDa
    ID gène
    23436
    UniProt
    P08861
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