Recombinant GFAP anticorps

N° du produit ABIN6939508

Cet anticorps Souris Monoclonal détecte spécifiquement GFAP dans WB, IHC, FACS et StM. Il présente une réactivité envers Humain, Rat, Souris, Porc, Boeuf (Vache), Poulet et Lapin.

Aperçu rapide pour Recombinant GFAP anticorps (ABIN6939508)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris, Porc, Boeuf (Vache), Poulet, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Clone: RASTRO-789

Détail du produit anti-GFAP anticorps

Specificité: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human GFAP protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Known Application: Flow Cytometry (1-2 μg/million cells),Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Poids moléculaire: ~50kDa

ID gène: 2670

UniProt: P14136