GLG1 anticorps

N° du produit ABIN6939532

Cet anticorps anti-GLG1 est un anticorps Souris Monoclonal détectant GLG1 dans IHC et StM. Adapté pour Humain.

Aperçu rapide pour GLG1 anticorps (ABIN6939532)

Antigène: GLG1 (Golgi Glycoprotein 1 (GLG1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GLG1 est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: GLG1-970

Détail du produit anti-GLG1 anticorps

Specificité: This MAb recognizes a protein of 134 kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Purified by Protein A/G

Immunogène: Golgi fraction from human liver cells

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur GLG1

Antigène: GLG1 (Golgi Glycoprotein 1 (GLG1))

Autre désignation: GLG1

Poids moléculaire: 134kDa

ID gène: 2734

UniProt: Q92896