GNRHR and LHCGR (AA 1-29), (N-Term) anticorps

N° du produit ABIN6939536

L’anticorps anti-GNRHR and LHCGR Monoclonal Souris est utilisé pour la détection de GNRHR and LHCGR dans des échantillons de Humain et Rat. Il a été validé pour FACS, IF, IHC et WB.

Aperçu rapide pour GNRHR and LHCGR (AA 1-29), (N-Term) anticorps (ABIN6939536)

Antigène: GNRHR and LHCGR

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: F1G4

Détail du produit

Épitope: AA 1-29, N-Term

Fonction: GnRH-Receptor / LH-RH Receptor Antibody

Specificité: Recognizes an epitope on the extracellular domain of gonadotropin releasing hormone (GnRH) receptor or luteinizing hormone receptor (LHCGR). Lutropin (also designated luteinizing hormone) plays a role in spermatogenesis and ovulation by stimulating the testes and ovaries to produce steroids. Gonadotropin (also designated choriogonadotropin) production in the placenta maintains estrogen and progesterone levels during the first trimester of pregnancy. Ovaries and testes abundantly express luteinizing hormone/choriogonadotropin receptor. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulating hormone (FSH). GnRH influences the protective effect of pregnancy and Gonadotropin against breast cancer. The expression of GnRH on breast carcinoma correlates in part to the degree of tumor differentiation. GnRH-positive breast tumors occur more frequently in tumors with greater cell differentiation in premenopausal women. GnRH is present in luteal and granulosa cells as well as in ovarian cell membrane preparations.

Réactivité croisée (Details): Predicted to react with Pig, Rabbit.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: A synthetic peptide aa 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) from the N-terminal of human GnRH receptor.

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: MCF-7, MDA231, T47D cells. Pituitary gland, ovarian or breast cancers.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: GNRHR and LHCGR

Sujet:

GnRH receptor, GnRH-R, GNRHR1, gonadotropin-releasing hormone (type 1) receptor 1, GRHR, HH7, leutinizing-releasing hormone receptor, LHRHR, LRHR, luliberin receptor, luteinizing hormone releasing hormone receptor, Type I GnRH receptor

MCF-7, MDA231, ovarian or breast cancers., T47D cells. Pituitary gland

Poids moléculaire: 54-60kDa

ID gène: 2798, 3973, 407587468490

UniProt: P30968