IGHA1 & IGHA2 anticorps

N° du produit ABIN6939703

Cet anticorps Souris Monoclonal détecte spécifiquement IGHA1 & IGHA2 dans FACS, IF, IHC et StM. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour IGHA1 & IGHA2 anticorps (ABIN6939703)

Antigène: IGHA1 & IGHA2

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: HISA43

Détail du produit

Specificité: This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with both IgA1 and IgA2 subclasses of Alpha heavy chain. It reacts with the third constant domain (CH3) of the alpha chain of IgA molecules. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins.IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Purification: Purified by Protein A/G

Immunogène: Purified human IgA

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL 30 min RT), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL 30 min RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: IGHA1 & IGHA2

Poids moléculaire: 50-75kDa

ID gène: 3493, 3494

UniProt: P01876, P01877