Recombinant IGHM anticorps

N° du produit ABIN6939721

Cet anticorps Souris Monoclonal détecte spécifiquement IGHM dans IHC et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant IGHM anticorps (ABIN6939721)

Antigène: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IGHM est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: RIM373

Détail du produit anti-IGHM anticorps

Specificité: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Purification: Purified by Protein A/G

Immunogène: Heavy chain of human IgM

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur IGHM

Antigène: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Autre désignation: IGHM

Poids moléculaire: 50-75kDa

ID gène: 3507

UniProt: P01871, P20769