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IgL anticorps

Cet anticorps anti-IgL est un anticorps Souris Monoclonal détectant IgL dans IHC, FACS, IF, WB et StM. Adapté pour Humain.
N° du produit ABIN6939763

Aperçu rapide pour IgL anticorps (ABIN6939763)

Antigène

IgL

Reactivité

Humain

Hôte

  • 9
  • 3
Souris

Clonalité

  • 11
  • 1
Monoclonal

Conjugué

  • 11
  • 1
Cet anticorp IgL est non-conjugé

Application

  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Staining Methods (StM)

Clone

N10-2
  • Specificité

    This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

    Purification

    Purified by Protein A/G

    Immunogène

    Purified IgG from human serum

    Isotype

    IgG1 kappa
  • Indications d'application

    Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

    Known Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions

    For Research Use only
  • Concentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-80 °C

    Stockage commentaire

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Date de péremption

    24 months
  • Antigène

    IgL

    Poids moléculaire

    ~22.5kDa

    ID gène

    3535, 3546

    UniProt

    P01701, P01842
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