Involucrin anticorps

N° du produit ABIN6939833

Cet anticorps anti-Involucrin est un anticorps Souris Monoclonal détectant Involucrin dans IHC, IF, FACS et StM. Adapté pour Humain, Chien, Porc, Gorilla et Owl Monkey.

Aperçu rapide pour Involucrin anticorps (ABIN6939833)

Antigène: Involucrin (IVL)

Reactivité: Humain, Chien, Porc, Gorilla, Owl Monkey

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Involucrin est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: SPM259

Détail du produit anti-Involucrin anticorps

Specificité: It recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Aucune reactivité croisée: Souris

Purification: Purified by Protein A/G

Immunogène: Purified involucrin from human keratinocytes

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Involucrin

Antigène: Involucrin (IVL)

Autre désignation: IVL

Poids moléculaire: 66-170kDa

ID gène: 3713

UniProt: P07476