Recombinant Involucrin anticorps

N° du produit ABIN6939835

L’anticorps Souris Monoclonal anti-Involucrin a été validé pour IHC, ISt et StM. Il convient pour détecter Involucrin dans des échantillons de Humain, Chien, Porc, Gorilla et Owl Monkey.

Aperçu rapide pour Recombinant Involucrin anticorps (ABIN6939835)

Antigène: Involucrin (IVL)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Chien, Porc, Gorilla, Owl Monkey

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Involucrin est non-conjugé

Application: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Clone: RIVRN-827

Détail du produit anti-Involucrin anticorps

Specificité: It recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Aucune reactivité croisée: Souris

Purification: Purified by Protein A/G

Immunogène: Recombinant human full-length Involucrin protein (IVRN)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37'C) (Note than enzyme digestion is better than citrate buffer based epitope unmasking),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Involucrin

Antigène: Involucrin (IVL)

Autre désignation: IVL

Poids moléculaire: 66-170kDa

ID gène: 3713

UniProt: P07476