KRT6A/KRT6B/KRT6C (C-Term) anticorps

N° du produit ABIN6939865

Détail du produit

Antigène: KRT6A/KRT6B/KRT6C

Épitope: C-Term

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunostaining (ISt)

Specificité: This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

Purification: Purified by Protein A/G

Immunogène: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6

Clone: SPM269

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: RAW, HeLa, Suprabasal cells of the outer hair follicle root sheath in skin. Basal cell carcinoma.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin (Sigma Cat. #P-7012) at 1 mg/mL Tris-HCl, pH 2.0 for 10-15 min at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: KRT6A/KRT6B/KRT6C

Autre désignation: KRT6A, KRT6B, KRT6C

Poids moléculaire: 56kDa

ID gène: 3853, 3854, 286887

UniProt: P02538, P04259, P48668