Cytokeratin 7 anticorps

N° du produit ABIN6939871

Détail du produit anti-Cytokeratin 7 anticorps

Antigène: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 7 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Specificité: It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Purification: Purified by Protein A/G

Immunogène: OTN 11, ovarian carcinoma cell line

Clone: OV-TL12-30

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Carcinoma of ovary, lung, cervix or breast.

Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cytokeratin 7

Antigène: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Autre désignation: KRT7 (KRT7 Produits)

Synonymes: anticorps CK7, anticorps K2C7, anticorps K7, anticorps SCL, anticorps D15Wsu77e, anticorps Krt2-7, anticorps KRT7, anticorps keratin 7, anticorps KRT7, anticorps Krt7

Poids moléculaire: 55kDa

ID gène: 3855

UniProt: P08729