Recombinant Keratin 10 anticorps

N° du produit ABIN6939901

Cet anticorps anti-Keratin 10 Monoclonal Souris (Clone rKRT10-1275) (ABIN6939901) détecte spécifiquement Keratin 10 dans IHC et WB. L’anticorps est réactif avec des échantillons de Humain, Chien et Cat.

Aperçu rapide pour Recombinant Keratin 10 anticorps (ABIN6939901)

Antigène: Keratin 10 (KRT10)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Chien, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 10 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: RKRT10-1275

Détail du produit anti-Keratin 10 anticorps

Fonction: Recombinant Cytokeratin 10 (Suprabasal Epithelial Marker) Antibody

Specificité: This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogène: Recombinant full-lenth human KRT10 protein.

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: A431, HeLa, MCF7 cells. Esophagus or tonsil.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Keratin 10

Antigène: Keratin 10 (KRT10)

Autre désignation: KRT10

Sujet:

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

A431, HeLa, MCF7 cells. Esophagus or tonsil. Skin.

Poids moléculaire: 56.5kDa

ID gène: 3858, 99936

UniProt: P13645