Recombinant EpCAM anticorps (N-Term)

N° du produit ABIN6939994

L’anticorps Lapin Monoclonal anti-EpCAM a été validé pour FACS, IHC et StM. Il convient pour détecter EpCAM dans des échantillons de Humain.

Aperçu rapide pour Recombinant EpCAM anticorps (N-Term) (ABIN6939994)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: EGP40-1555R

Détail du produit anti-EpCAM anticorps

Épitope: AA 20-60, N-Term

Specificité: Recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (EpCAM). EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Aucune reactivité croisée: Souris, Rat (Rattus)

Immunogène: A synthetic peptide (around aa 20-60) from the N-terminus of human TACSTD1 protein

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: MCF-7 or HT29 cells. Breast tumor.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: TACSTD1

Poids moléculaire: 40-43kDa

ID gène: 4072

UniProt: P16422