Recombinant EpCAM anticorps (Extracellular Domain)

N° du produit ABIN6939997

L’anticorps anti-EpCAM Monoclonal Lapin est utilisé pour la détection de EpCAM dans des échantillons de Humain. Il a été validé pour FACS et IHC.

Aperçu rapide pour Recombinant EpCAM anticorps (Extracellular Domain) (ABIN6939997)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: EGP40-2571R

Détail du produit anti-EpCAM anticorps

Épitope: AA 100-224, Extracellular Domain

Fonction: Recombinant Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody

Specificité: Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Purification: 200ug/ml of Ab Purified by Protein A.

Immunogène: Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)

Isotype: IgG, kappa

Information d'application

Indications d'application:

Positive Control: MCF-7 or HT29 cells. Breast tumor.

Known Application: Flow Cytometry (1-2 μg/million cells). Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: TACSTD1

Sujet:

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

MCF-7 or HT29 cells. Breast tumor.

Poids moléculaire: 40-43kDa

ID gène: 4072, 542050

UniProt: P16422