CD99 anticorps

N° du produit ABIN6940046

Cet anticorps anti-CD99 est un anticorps Lapin Polyclonal détectant CD99 dans IHC et StM. Adapté pour Humain.

Aperçu rapide pour CD99 anticorps (ABIN6940046)

Antigène: CD99

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD99 est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Détail du produit anti-CD99 anticorps

Specificité: Recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.

Immunogène: Recombinant human MIC2 protein

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: MOLT-4 cells. Pancreas or Ewing's sarcoma.

Known Application: Immunohistochemistry (Formalin-fixed) (5-10 μg/mL for 30 min at Room Temp)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD99

Antigène: CD99

Autre désignation: CD99

Poids moléculaire: 27-32kDa

ID gène: 4267

UniProt: P14209