MTAP anticorps (AA 97-196)

N° du produit ABIN6940087

Cet anticorps Souris Monoclonal détecte spécifiquement MTAP dans WB, IHC, ELISA, Coat et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour MTAP anticorps (AA 97-196) (ABIN6940087)

Antigène: MTAP (Methylthioadenosine phosphorylase (MTAP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MTAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Clone: MTAP-1813

Détail du produit anti-MTAP anticorps

Épitope: AA 97-196

Specificité: Recognizes a protein of 31 kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

Purification: Purified by Protein A/G

Immunogène: Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: HepG2, A431, HeLa, HAP1 or MCF-7 cells. Kidney.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MTAP

Antigène: MTAP (Methylthioadenosine phosphorylase (MTAP))

Autre désignation: MTAP

Poids moléculaire: 31kDa

ID gène: 4507

UniProt: Q13126

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process