Recombinant MUC1 anticorps

N° du produit ABIN6940113

Cet anticorps anti-MUC1 est un anticorps Lapin Monoclonal détectant MUC1 dans IHC, ELISA, Coat et StM. Adapté pour Humain et Souris.

Aperçu rapide pour Recombinant MUC1 anticorps (ABIN6940113)

Antigène: MUC1 (Mucin 1 (MUC1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC1 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Clone: MUC1-2818R

Détail du produit anti-MUC1 anticorps

Specificité: This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in 90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Purification: Purified by Protein A/G

Immunogène: Human milk-fat globule membranes (HMFGM)

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Known Application: ELISA (For coating use Ab at 2-5 μg/mL, order Ab without BSA) (Very good capturing Ab), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MUC1

Antigène: MUC1 (Mucin 1 (MUC1))

Autre désignation: MUC1

Poids moléculaire: 265-400kDa

ID gène: 4582

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling