Geminin anticorps

N° du produit ABIN6940265

Cet anticorps Souris Monoclonal détecte spécifiquement Geminin dans IHC, IF et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour Geminin anticorps (ABIN6940265)

Antigène: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Geminin est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Clone: CPTC-GMMN-1

Détail du produit anti-Geminin anticorps

Purification: Purified by Protein A/G

Immunogène: Recombinant human full-length protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HEK293, HeLa, MCF-7, Tonsil, Triple-negative breast cancer.

Known Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Geminin

Antigène: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Autre désignation: GMMN

Sujet: Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphasepromoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitinmediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

Poids moléculaire: 35kDa

ID gène: 51053

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication