Geminin anticorps

N° du produit ABIN6940265

Détail du produit anti-Geminin anticorps

Antigène: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Geminin est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Recombinant human full-length protein

Clone: CPTC-GMMN-1

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HEK293, HeLa, MCF-7, Tonsil, Triple-negative breast cancer.

Known Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Geminin

Antigène: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Autre désignation: GMMN (GMNN Produits)

Synonymes: anticorps CG3183, anticorps Dm Geminin, anticorps Dm geminin, anticorps Dmel\\CG3183, anticorps Gem, anticorps Geminin, anticorps anon-WO0257455.1, anticorps gem, anticorps l(2)k14019, anticorps v(2)k09107, anticorps sb:cb254, anticorps zgc:66217, anticorps GB16871, anticorps GMNN, anticorps geminin, anticorps dyak_GLEANR_833, anticorps DyakGE24654, anticorps GE24654, anticorps LOC100049517, anticorps AW546347, anticorps geminin, DNA replication inhibitor, anticorps CG3183 gene product from transcript CG3183-RA, anticorps geminin, anticorps GE24654 gene product from transcript GE24654-RA, anticorps GMNN, anticorps geminin, anticorps gmnn, anticorps LOC410093, anticorps Dyak\geminin, anticorps Gmnn

Sujet: Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphasepromoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitinmediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

Poids moléculaire: 35kDa

ID gène: 51053

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication