POLR2A/RPB1 anticorps (pSer5)

N° du produit ABIN6940331

L’anticorps Souris Monoclonal anti-POLR2A/RPB1 a été validé pour WB, IHC, IF, FACS et StM. Il convient pour détecter POLR2A/RPB1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour POLR2A/RPB1 anticorps (pSer5) (ABIN6940331)

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp POLR2A/RPB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: CTD4H8

Détail du produit anti-POLR2A/RPB1 anticorps

Épitope: pSer5

Purification: Purified by Protein A/G

Immunogène: Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur POLR2A/RPB1

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Autre désignation: POLR2A

Sujet: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Poids moléculaire: 192-253kDa

ID gène: 5430

UniProt: P24928

Pathways: Regulatory RNA Pathways