MAML3 anticorps

N° du produit ABIN6940389

L’anticorps Souris Monoclonal anti-MAML3 a été validé pour ISt, IHC et StM. Il convient pour détecter MAML3 dans des échantillons de Humain.

Aperçu rapide pour MAML3 anticorps (ABIN6940389)

Antigène: MAML3 (Mastermind-Like 3 (MAML3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAML3 est non-conjugé

Application: Immunostaining (ISt), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: MAML3-1303

Détail du produit anti-MAML3 anticorps

Purification: Purified by Protein A/G

Immunogène: Recombinant human MAML3 protein fragment

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MAML3

Antigène: MAML3 (Mastermind-Like 3 (MAML3))

Autre désignation: MAML3

Sujet: MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.

Poids moléculaire: 150-170kDa

ID gène: 55534

UniProt: Q96JK9