Spastin anticorps

N° du produit ABIN6940629

L’anticorps Souris Monoclonal anti-Spastin a été validé pour WB, ELISA, IHC, IF, Coat et StM. Il convient pour détecter Spastin dans des échantillons de Souris, Rat et Humain.

Aperçu rapide pour Spastin anticorps (ABIN6940629)

Antigène: Spastin (SPAST)

Reactivité: Souris, Rat, Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Spastin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

Clone: Sp 3G11-1

Détail du produit anti-Spastin anticorps

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human Spastin protein.

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Rat brain extract.

Known Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Spastin

Antigène: Spastin (SPAST)

Autre désignation: SPAST

Sujet: The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs.

Poids moléculaire: 52kDa

ID gène: 6683

Pathways: Dynamique des Microtubules, M Phase, Regulation of Cell Size