Recombinant CD43 anticorps

N° du produit ABIN6940641

Cet anticorps Lapin Monoclonal détecte spécifiquement CD43 dans FACS, WB, IHC, IF et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant CD43 anticorps (ABIN6940641)

Antigène: CD43 (SPN) (Sialophorin (SPN))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD43 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Clone: SPN-1766R

Détail du produit anti-CD43 anticorps

Specificité: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human SPN protein

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: K562 cells Paracortex in a tonsil or a reactive lymph node.

Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD43

Antigène: CD43 (SPN) (Sialophorin (SPN))

Autre désignation: SPN

Poids moléculaire: 95, 115, or 135kDa

ID gène: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity