Transgelin anticorps

N° du produit ABIN6940678

Cet anticorps anti-Transgelin est un anticorps Souris Monoclonal détectant Transgelin dans IHC et StM. Adapté pour Humain, Souris, Lapin, Boeuf (Vache) et Porc.

Aperçu rapide pour Transgelin anticorps (ABIN6940678)

Antigène: Transgelin (TAGLN)

Reactivité: Humain, Souris, Lapin, Boeuf (Vache), Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transgelin est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: TAGLN-247

Détail du produit anti-Transgelin anticorps

Specificité: This MAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human transgelin (TAGLN) protein.

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Transgelin

Antigène: Transgelin (TAGLN)

Autre désignation: TAGLN

Poids moléculaire: 22kDa

ID gène: 6876

UniProt: Q01995