Transferrin anticorps (AA 311-445)

N° du produit ABIN6940685

Cet anticorps Souris Monoclonal détecte spécifiquement Transferrin dans ELISA, IHC, Coat et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour Transferrin anticorps (AA 311-445) (ABIN6940685)

Antigène: Transferrin (TF)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Clone: TF-3001

Détail du produit anti-Transferrin anticorps

Épitope: AA 311-445

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (around aa 311-445) of human TF protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Jurkat, HeLa, MCF-7 or K562 cells. Human liver.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Transferrin

Antigène: Transferrin (TF)

Autre désignation: Transferrin (Early Marker of Oligodendrocytes)

Sujet: Iron (Fe) is a tightly metabolically controlled mineral and growth factor present in all living cells. Iron not bound in erythrocyte hemoglobin is transported by transferrin (Tf), the iron transport protein of vertebrate serum. The transferrin protein contains two homologous domains, each of which contain an Fe-binding site. The majority of transferrin is synthesized in the liver and secreted into the blood, but it is also produced in lower amounts in testis and brain as well as in oligodendrocytes, where transferrin is an early marker of oligodendrocyte differentiation. From the blood, transferrin is internalized by erythroblasts and reticulocytes upon binding the transferrin receptor (TfR), also designated CD71, through a system of coated pits and vesicles. After Fe release, transferrin is returned to the extracellular medium, where it can be reused. Defects in the transferrin gene results in atransferrinemia, a rare autosomal recessive disorder characterized by microcytic anemia and iron loading.

Poids moléculaire: 79kDa

ID gène: 7018

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis