Transglutaminase 2 anticorps

N° du produit ABIN6940725

L’anticorps anti-Transglutaminase 2 Monoclonal Souris est utilisé pour la détection de Transglutaminase 2 dans des échantillons de Humain, Souris, Rat, Singe et Lapin. Il a été validé pour WB et IHC.

Aperçu rapide pour Transglutaminase 2 anticorps (ABIN6940725)

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivité: Humain, Souris, Rat, Singe, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transglutaminase 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: TGM2-419

Détail du produit anti-Transglutaminase 2 anticorps

Fonction: Transglutaminase II (TGM2) Antibody

Specificité: Recognizes a 77-85 kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human TGM2 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: HUVEC cells. Endothelial cells in placenta, liver, brain or breast carcinoma. Smooth muscle cells of any origin (e. G. Intestine).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL, 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Transglutaminase 2

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Autre désignation: TGM2

Sujet:

Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGase C, TGase H, TGase-2, TgaseII, TGC, TGM2, Tissue type transglutaminase, Transglutaminase 2 C polypeptide, Transglutaminase H, tTG, tTGas

Brain, HUVEC cells. Endothelial cells in placenta, Liver, or breast carcinoma. Smooth muscle cells of any origin

Poids moléculaire: 77-85kDa

ID gène: 7052, 517033

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling